By M. Ahearne, Alicia J. El Haj, S. Rauz (auth.), Alicia El Haj, Dan Bader (eds.)
This quantity offers chosen peer-reviewed papers of the eighth foreign convention on mobile & Stem mobilephone Engineering (ICCM) 2010 in Dublin. The contributions are written via best scientists in mobile and Stem phone Engineering and the themes of the papers comprise:
Computational mobilephone Mechanics
Experimental thoughts in phone Mechanics
Molecular and mobilephone Imaging
Cell Matrix Interactions
Mechanotransduction and telephone mechanics
Stem mobile area of interest
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Additional info for 8th International Conference on Cell & Stem Cell Engineering (ICCE)
Taking into account sGAG both accumulated and released per construct over the 42 day culture period (Fig. 1F), we observe a trend towards greater sGAG synthesis in microchanneled constructs, when normalized to DNA. However total sGAG accumulation was lower in this group. This indicates that while the introduction of microchannels may lead to greater ECM synthesis in constructs, the additional ECM components seem to be lost to the surrounding media and not retained by the constructs. Overall the total sGAG accumulation in the construct is reduced by the introduction of channels.
Human embryonic stem cells (hESC) cannot be propagated on TCPS at all and require feeder layers or MatrigelTM, both of which introduce a xenogenic threat. Obviously, these platforms do not emulate the physiological stem cell microenvironment, consisting of soluble factors, neighbouring cells and a complex extracellular matrix (ECM). We directed ECM deposition by human fibroblasts through application of macromolecular crowding followed by detergent lysis to achieve a cell-free matrix. Long-term propagation of MSCs for 58 days on these bioassembled matrices increased their population doublings by 79% compared to TCPS controls.
Constructs were loaded each day for 2 consecutive hours, 5days/week over 42 days. Free swelling (FS) controls were maintained adjacent to the bioreactor during loading periods, in the same amount of medium. Both solid and microchanneled constructs were subjected to both culturing regimes, and constructs were assessed at 0, 21, and 42 days. 05 M EDTA, pH 6 (all SigmaAldrich, Ireland) under constant rotation at 60ºC for 18 hours. DNA content was quantified using the Hoechst Bisbenzimide 33258 dye assay.
8th International Conference on Cell & Stem Cell Engineering (ICCE) by M. Ahearne, Alicia J. El Haj, S. Rauz (auth.), Alicia El Haj, Dan Bader (eds.)